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Title:

Two-photon excitation with pico-second fluorescence lifetime imaging to detect nuclear association of flavanols

Document type:
Zeitschriftenaufsatz
Author(s):
Mueller-Harvey, Irene; Feucht, Walter; Polster, Juergen; Trnková, Lucie; Burgos, Pierre; Parker, Anthony W.; Botchway, Stanley W.
Abstract:
Two-photon excitation enabled for the first time the observation and measurement of excited state fluorescence lifetimes from three flavanols in solution, which were {̃{}}1.0 ns for catechin and epicatechin, but {\textless}45 ps for epigallocatechin gallate (EGCG). The shorter lifetime for EGCG is in line with a lower fluorescence quantum yield of 0.003 compared to catechin (0.015) and epicatechin (0.018). In vivo experiments with onion cells demonstrated that tryptophan and quercetin, which ten...     »
Keywords:
Catechin/analogs {&} derivatives/analysis; Flavonols/analysis; Microscopy, Fluorescence, Multiphoton; Onions/chemistry/ultrastructure; Spectrometry, Fluorescence; Taxus/chemistry
Journal title:
Analytica chimica acta
Year:
2012
Journal volume:
719
Pages contribution:
68--75
Fulltext / DOI:
doi:10.1016/j.aca.2011.12.068
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