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Title:

Flavanol binding of nuclei from tree species

Document type:
Zeitschriftenaufsatz
Author(s):
Feucht, W.; Treutter, D.; Polster, J.
Abstract:
Light microscopy was used to examine the nuclei of five tree species with respect to the presence of flavanols. Flavanols develop a blue colouration in the presence of a special p-dimethylaminocinnamaldehyde (DMACA) reagent that enables those nuclei loaded with flavanols to be recognized. Staining of the nuclei was most pronounced in both Tsuga canadensis and Taxus baccata, variable in Metasequoia glyptostroboides, faint in Coffea arabica and minimal in Prunus avium. HPLC analysis showed that th...     »
Keywords:
Binding Sites; Catechin/metabolism; Cell Nucleus/metabolism; Chromosomes, Plant/metabolism; Coffea/metabolism; Flavonols/metabolism; Plant Leaves/metabolism; Prunus/metabolism; Sensitivity and Specificity; Sequoia/metabolism; Species Specificity; Taxus/metabolism; Trees/classification/metabolism; Tsuga/metabolism; Vacuoles/metabolism/ultrastructure
Journal title:
Plant cell reports
Year:
2004
Journal volume:
22
Journal issue:
6
Pages contribution:
430--436
Fulltext / DOI:
doi:10.1007/s00299-003-0705-7
Print-ISSN:
0721-7714
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