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Title:

Detecting potato viruses using direct reverse transcription quantitative PCR (DiRT-qPCR) without RNA purification: An alternative to DAS-ELISA

Document type:
Zeitschriftenaufsatz
Author(s):
Stammler, Johanna; Oberneder, Anita; Kellermann, Adolf; Hadersdorfer, Johannes
Abstract:
Virus screening is obligatory to avoid the spread of plant viruses regionally and globally. Double-antibody sandwich (DAS)-ELISA is the standard for screening potato viruses owing to its high-throughput potential, robustness, and cost--benefit ratio. However, low virus titers present in dormant potato tubers may not be reliably detected by using DAS-ELISA. Virus enrichment for reliable virus detection by DAS-ELISA assay is time-consuming and can be avoided by switching to more sensitive molecula...     »
Journal title:
European Journal of Plant Pathology
Year:
2018
Journal volume:
97
Journal issue:
1
Pages contribution:
119
Fulltext / DOI:
doi:10.1007/s10658-018-1468-x
Print-ISSN:
0929-1873
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