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Original title:
Genexpressionsanalyse von Komponenten des Urokinase-Systems im Sphäroid-Mikrotumormodell
Translated title:
Gene expression analysis of components of the urokinase plasminogen activator system in microtumor-like 3D spheroids
Author:
Kohl, Christina Elisabeth
Year:
2018
Document type:
Dissertation
Faculty/School:
Fakultät für Medizin
Advisor:
Magdolen, Viktor (Prof. Dr.)
Referee:
Magdolen, Viktor (Prof. Dr.); Kiechle, Marion B. (Prof. Dr.); Bassermann, Florian (Prof. Dr.)
Language:
de
Subject group:
MED Medizin
TUM classification:
MED 560d
Abstract:
Dreidimensionale (3D) Sphäroide spiegeln aufgrund ihrer Zell-Zell-Kontakte tumorassoziierte Prozesse besser wider als 2D-Monolayerzellen. Mittels qPCR wurde die mRNA Expression verschiedener tumorrelevanter Faktoren (uPA, uPAR, PAI-1, KLK7, Rab31) in 3D versus 2D kultivierten Zellen verschiedener Tumorentitäten untersucht und in vielen Fällen eine differentielle Genexpression in den Mikrotumor-ähnlichen Sphäroiden beobachtet, was die Bedeutung von 3D-Sphäroiden in der Tumorforschung unterstreich...     »
Translated abstract:
Due to their three-dimensional cell-cell interactions, spheroids better reflect tumor-associated processes than 2D monolayer cells. mRNA expression of several tumor-relevant factors (uPA, uPAR, PAI-1, KLK7, Rab31) was analyzed via qPCR analysis in 3D versus 2D cultivated cells of different tumor entities. In many cases, differential gene expression was observed in microtumor-like spheroids compared to 2D- cultivated cells. The results highlight the importance of 3D spheroids in tumor research.
WWW:
https://mediatum.ub.tum.de/?id=1391212
Date of submission:
04.10.2017
Oral examination:
04.07.2018
File size:
1859121 bytes
Pages:
126
Urn (citeable URL):
https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20180704-1391212-1-0
Last change:
05.09.2018
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