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- Title:
Near-infrared branding efficiently correlates light and electron microscopy.
- Document type:
- Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
- Author(s):
- Bishop, Derron; Niki?, Ivana; Brinkoetter, Mary; Knecht, Sharmon; Potz, Stephanie; Kerschensteiner, Martin; Misgeld, Thomas
- Abstract:
- The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.
- Journal title abbreviation:
- Nat Methods
- Year:
- 2011
- Journal volume:
- 8
- Journal issue:
- 7
- Pages contribution:
- 568-70
- Language:
- eng
- Fulltext / DOI:
- doi:10.1038/nmeth.1622
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/21642966
- Print-ISSN:
- 1548-7091
- TUM Institution:
- Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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