Near-infrared branding efficiently correlates light and electron microscopy.

Title:: Near-infrared branding efficiently correlates light and electron microscopy.
Document type:: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Author(s):: Bishop, Derron; Niki?, Ivana; Brinkoetter, Mary; Knecht, Sharmon; Potz, Stephanie; Kerschensteiner, Martin; Misgeld, Thomas
Abstract:: The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.
Journal title abbreviation:: Nat Methods
Year:: 2011
Journal volume:: 8
Journal issue:: 7
Pages contribution:: 568-70
Language:: eng
Fulltext / DOI:: doi:10.1038/nmeth.1622
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/21642966
Print-ISSN:: 1548-7091
TUM Institution:: Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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