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Titel:

Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo.

Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't; Article
Autor(en):
Garaschuk, O; Milos, RI; Konnerth, A
Abstract:
One of the challenges for modern neuroscience is to understand the rules of concerted neuronal function in vivo. This question can be addressed using noninvasive high-resolution imaging techniques like two-photon microscopy. This protocol describes a versatile approach for in vivo two-photon calcium imaging of neural networks, stained with membrane-permeant fluorescent-indicator dyes. It is based on a targeted pressure ejection of the dye into the tissue of interest and can be used for a large s...     »
Zeitschriftentitel:
Nat Protoc
Jahr:
2006
Band / Volume:
1
Heft / Issue:
1
Seitenangaben Beitrag:
380-6
Sprache:
eng
Volltext / DOI:
doi:10.1038/nprot.2006.58
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/17406260
Print-ISSN:
1754-2189
TUM Einrichtung:
Institut für Neurowissenschaften
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