User: Guest  Login
Less Searchfields
Simple search
Title:

Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo.

Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article
Author(s):
Garaschuk, O; Milos, RI; Konnerth, A
Abstract:
One of the challenges for modern neuroscience is to understand the rules of concerted neuronal function in vivo. This question can be addressed using noninvasive high-resolution imaging techniques like two-photon microscopy. This protocol describes a versatile approach for in vivo two-photon calcium imaging of neural networks, stained with membrane-permeant fluorescent-indicator dyes. It is based on a targeted pressure ejection of the dye into the tissue of interest and can be used for a large s...     »
Journal title abbreviation:
Nat Protoc
Year:
2006
Journal volume:
1
Journal issue:
1
Pages contribution:
380-6
Language:
eng
Fulltext / DOI:
doi:10.1038/nprot.2006.58
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/17406260
Print-ISSN:
1754-2189
TUM Institution:
Institut für Neurowissenschaften
 BibTeX