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Title:

"In vivo" monitoring of neuronal network activity in zebrafish by two-photon Ca(2+) imaging.

Document type:
Journal Article; Article
Author(s):
Brustein, E; Marandi, N; Kovalchuk, Y; Drapeau, P; Konnerth, A
Abstract:
The zebrafish larva is a powerful model for the analysis of behaviour and the underlying neuronal network activity during early stages of development. Here we employ a new approach of "in vivo" Ca(2+) imaging in this preparation. We demonstrate that bolus injection of membrane-permeable Ca(2+) indicator dyes into the spinal cord of zebrafish larvae results in rapid staining of essentially the entire spinal cord. Using two-photon imaging, we could monitor Ca(2+) signals simultaneously from a larg...     »
Journal title abbreviation:
Pflugers Arch
Year:
2003
Journal volume:
446
Journal issue:
6
Pages contribution:
766-73
Language:
eng
Fulltext / DOI:
doi:10.1007/s00424-003-1138-4
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/12883893
Print-ISSN:
0031-6768
TUM Institution:
Institut für Neurowissenschaften
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