Optical imaging of experimental arthritis using allogeneic leukocytes labeled with a near-infrared fluorescent probe.

Simon, GH; Daldrup-Link, HE; Kau, J; Metz, S; Schlegel, J; Piontek, G; Saborowski, O; Demos, S; Duyster, J; Pichler, BJ

doi:10.1007/s00259-006-0081-y

Benutzer: Gast

journal article

Titel:: Optical imaging of experimental arthritis using allogeneic leukocytes labeled with a near-infrared fluorescent probe.
Dokumenttyp:: Journal Article
Autor(en):: Simon, GH; Daldrup-Link, HE; Kau, J; Metz, S; Schlegel, J; Piontek, G; Saborowski, O; Demos, S; Duyster, J; Pichler, BJ
Abstract:: PURPOSE: The purpose of this study was to assess the feasibility of inflammation detection in an antigen-induced arthritis model using fluorescent leukocytes and optical imaging. METHODS: Antigen-mediated monoarthritis was induced in the right knee of 12 Sprague-Dawley rats. Six rats remained untreated and six rats were treated with cortisone. All rats received ex vivo fluorescent-labeled rat leukocytes. Optical images of both knees were acquired before and at 5 min, 1 h, 4 h, and 24 h after cell injection. Images were evaluated qualitatively and quantitatively by calculating signal intensity ratios between the right arthritic (A) and contralateral normal (N) knee. A/N ratios were tested for significant differences between baseline values and values after cell injection using a paired t test as well as between the untreated and cortisone-treated group using an unpaired t test. Synovial specimens were processed and evaluated for labeled cells with fluorescence microscopy. RESULTS: At 4 h and 24 h p.i., the A/N ratios of untreated arthritic knees showed a significant signal increase compared with baseline values (p<0.05) and a significant difference compared with A/N ratios of cortisone-treated animals (p<0.05). Fluorescent microscopy confirmed the presence of labeled cells in the arthritic synovium. CONCLUSION: Inflammation in antigen-induced arthritis can be detected with ex vivo labeled allogenic leukocytes and optical imaging.
Zeitschriftentitel:: Eur J Nucl Med Mol Imaging
Jahr:: 2006
Band / Volume:: 33
Heft / Issue:: 9
Seitenangaben Beitrag:: 998-1006
Sprache:: eng
Volltext / DOI:: doi:10.1007/s00259-006-0081-y
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/16770602
Print-ISSN:: 1619-7070
TUM Einrichtung:: III. Medizinische Klinik und Poliklinik (Hämatologie / Onkologie); Institut für Allgemeine Pathologie und Pathologische Anatomie
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Preclinical Medicine Institut für Allgemeine Pathologie und Pathologische Anatomie (Dr. Mogler komm.)2006

mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Clinical Medicine Klinik und Poliklinik für Innere Medizin III, Hämatologie und Onkologie (Prof. Bassermann)2006