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Title:

Rapid adaptive optical recovery of optimal resolution over large volumes.

Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article
Author(s):
Wang, Kai; Milkie, Daniel E; Saxena, Ankur; Engerer, Peter; Misgeld, Thomas; Bronner, Marianne E; Mumm, Jeff; Betzig, Eric
Abstract:
Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafi...     »
Journal title abbreviation:
Nat Methods
Year:
2014
Journal volume:
11
Journal issue:
6
Pages contribution:
625-8
Language:
eng
Fulltext / DOI:
doi:10.1038/nmeth.2925
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/24727653
Print-ISSN:
1548-7091
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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