In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats.

Titel:: In vivo protein-protein interaction studies with BiFC: conditions, cautions, and caveats.
Dokumenttyp:: journal article
Autor(en):: Boevink, Petra, P; McLellan, Hazel, H; Bukharova, Tatyana, T; Engelhardt, Stefan, S; Birch, Paul, P
Abstract:: Bimolecular fluorescence complementation (BiFC), performed with suitable controls and the right conditions, can be a straightforward and simple method to assess protein-protein interactions accessible to anyone with basic confocal microscopy skills. It is of course not without its own potential pitfalls and requires specific controls. Here we describe its use to study the interactions between pathogen effector proteins and host proteins inside plant cells.
Zeitschriftentitel:: Methods Mol Biol
Jahr:: 2014
Band / Volume:: 1127
Seitenangaben Beitrag:: 81-90
Sprache:: eng
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/24643553
Print-ISSN:: 1064-3745
TUM Einrichtung:: Institut für Pharmakologie und Toxikologie
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