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Title:

Neurons on tape: Automated Tape Collecting Ultramicrotomy-mediated volume EM for targeting neuropathology.

Document type:
Review; Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Kislinger, Georg; Niemann, Cornelia; Rodriguez, Lucia; Jiang, Hanyi; Fard, Maryam K; Snaidero, Nicolas; Schumacher, Adrian-Minh; Kerschensteiner, Martin; Misgeld, Thomas; Schifferer, Martina
Abstract:
In this chapter, we review Automated Tape Collecting Ultramicrotomy (ATUM), which, among other array tomography methods, substantially simplified large-scale volume electron microscopy (vEM) projects. vEM reveals biological structures at nanometer resolution in three dimensions and resolves ambiguities of two-dimensional representations. However, as the structures of interest-like disease hallmarks emerging from neuropathology-are often rare but the field of view is small, this can easily turn a...     »
Journal title abbreviation:
Methods Cell Biol
Year:
2023
Journal volume:
177
Pages contribution:
125-170
Fulltext / DOI:
doi:10.1016/bs.mcb.2023.01.012
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/37451765
Print-ISSN:
0091-679X
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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