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Titel:

LOTOS-based two-photon calcium imaging of dendritic spines in vivo.

Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't; Article
Autor(en):
Chen, X; Leischner, U; Varga, Z; Jia, H; Deca, D; Rochefort, NL; Konnerth, A
Abstract:
Neurons in the mammalian brain receive thousands of synaptic inputs on their dendrites. In many types of neurons, such as cortical pyramidal neurons, excitatory synapses are formed on fine dendritic protrusions called spines. Usually, an individual spine forms a single synaptic contact with an afferent axon. In this protocol, we describe a recently established experimental procedure for measuring intracellular calcium signals from dendritic spines in cortical neurons in vivo by using a combinati...     »
Zeitschriftentitel:
Nat Protoc
Jahr:
2012
Band / Volume:
7
Heft / Issue:
10
Seitenangaben Beitrag:
1818-29
Sprache:
eng
Volltext / DOI:
doi:10.1038/nprot.2012.106
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/22976353
Print-ISSN:
1754-2189
TUM Einrichtung:
Institut für Neurowissenschaften
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