Signaling mechanisms involved in the intestinal pro-secretory actions of hydrogen sulfide.

Krueger, D; Foerster, M; Mueller, K; Zeller, F; Slotta-Huspenina, J; Donovan, J; Grundy, D; Schemann, M

doi:10.1111/j.1365-2982.2010.01571.x

Benutzer: Gast

journal article

Titel:: Signaling mechanisms involved in the intestinal pro-secretory actions of hydrogen sulfide.
Dokumenttyp:: Journal Article
Autor(en):: Krueger, D; Foerster, M; Mueller, K; Zeller, F; Slotta-Huspenina, J; Donovan, J; Grundy, D; Schemann, M
Abstract:: Background H(2) S actions in the gut involve neural activation. This study aimed to reveal the signaling mechanisms responsible for the pro-secretory effect of H(2) S by using TRPV1 and unselective TRP blockers and inhibitors of other signaling cascades hitherto described to be targeted by H(2) S elsewhere. Methods Ussing chamber voltage clamp technique was used to study actions of the H(2) S donor NaHS on secretion in guinea-pig and human colon. NaHS effects on guinea-pig primary afferents were also evaluated. Key Results NaHS evoked secretion was significantly reduced in guinea-pig and human tissue by the selective TRPV1 blockers capsazepine, AMG9801, SB705498, BCTC; LY294002 (Phosphatidylinositol-3 kinase (PI3K) inhibitor), SKF96365 (store operated calcium channel blocker), 2-APB (inositol triphosphate blocker), and atropine but not by HC030031 (TRPA1 blocker) or L- and T-type calcium channel antagonists. Actions of TRPV1 antagonists suggested non-competitive inhibition at multiple sites. In guinea-pig colon, Gd(3+) and La(3+) (unselective TRP blockers) had no effects while ruthenium red reduced NaHS effects; in human colon Gd(3+) attenuated NaHS response. NaHS response was inhibited by neurokinin-1 and -3 receptor blockers in guinea-pig and neurokinin-1 and -2 receptor blockade in human tissue. There was cross-desensitization between NaHS and capsaicin responses. NaHS induced capsazepine and LY294002 sensitive afferent discharge. Conclusions & Inferences H(2) S evokes mucosal secretion by targeting TRPV1 expressing afferent nerves which activate cholinergic secretomotor neurons via release of substance P acting in a species dependent manner on neurokinin-1, -2 or -3 receptors. Besides TRPV1 signaling H(2) S may target intracellular calcium dependent pathways and PI3K.
Zeitschriftentitel:: Neurogastroenterol Motil
Jahr:: 2010
Band / Volume:: 22
Heft / Issue:: 11
Seitenangaben Beitrag:: 1224-e320
Sprache:: eng
Volltext / DOI:: doi:10.1111/j.1365-2982.2010.01571.x
PubMed:: http://view.ncbi.nlm.nih.gov/pubmed/20659296
Print-ISSN:: 1350-1925
TUM Einrichtung:: Institut für Allgemeine Pathologie und Pathologische Anatomie
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mediaTUM Gesamtbestand Hochschulbibliographie 2010 Fakultäten Medizin Institut für Allgemeine Pathologie und Pathologische Anatomie

mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Preclinical Medicine Institut für Allgemeine Pathologie und Pathologische Anatomie (Dr. Mogler komm.)2010