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- Title:
Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons.
- Document type:
- Journal Article
- Author(s):
- Hees, J Tabitha; Harbauer, Angelika B
- Abstract:
- The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging.
- Journal title abbreviation:
- Methods Mol Biol
- Year:
- 2022
- Journal volume:
- 2431
- Pages contribution:
- 225-237
- Fulltext / DOI:
- doi:10.1007/978-1-0716-1990-2_11
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/35412279
- Print-ISSN:
- 1064-3745
- TUM Institution:
- Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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