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- Title:
Influence of different types of exercise on the expression of haem oxygenase-1 in leukocytes
- Document type:
- Zeitschriftenaufsatz
- Author(s):
- Fehrenbach, E.; Niess, A. M.; Passek, F.; Sorichter, S.; Schwirtz, A.; Berg, A.; Dickhuth, H. H.; Northoff, H.
- Abstract:
- Haem-oxygenase-1 (HO-1) is an antioxidant stress protein that is mainly induced by reactive oxygen species, inflammatory cytokines and hyperthermia. We assessed the influence of different types of exercise on HO-1 expression in leukocytes of the peripheral blood in three groups of male participants: a short exhaustive run above the lactate steady state (n = 15), eccentric exercise (n = 12) and an intensive endurance run (half-marathon, n = 12). Blood samples were taken at rest and up to 24 h after exercise. Blood lactate concentration after exercise was 9.0 +/- 2.1, 3.8 +/- 1.6 and 5.1 +/- 2.2 mmol x l(-1) (mean +/- s) for the exhaustive run, eccentric exercise and half-marathon groups, respectively (P < 0.05). Creatine kinase concentration was highest 24 h after exercise: 133 +/- 91, 231 +/- 139 and 289 +/- 221 U x l(-1) for the exhaustive run, eccentric exercise and half-marathon groups, respectively (P < 0.05). The maximal increase in leukocyte counts after exercise was 11.5 +/- 19.2, 6.2 +/- 1.4 and 14.7 +/- 2.1 x 10(9) x l(-1). There was no change in HO-1 as a result of the short exhaustive run or the eccentric exercise, whereas the half-marathon had a significant stimulatory effect on HO-1-expression in lymphocytes, monocytes and granulocytes (P < 0.001) using flow cytometry analyses. In conclusion, eccentric exercise alone or short-term heavy exercise are not sufficient to stimulate the antioxidative stress protein HO-1 in peripheral leukocytes
- Keywords:
- Adult; Creatine Kinase/blood; Exercise/*physiology; Heme Oxygenase (Decyclizing)/*metabolism; Heme Oxygenase-1; Humans; Lactic Acid/blood; Leukocyte Count; Leukocytes/*enzymology; Male; Membrane Proteins; Physical Endurance/physiology; Running/physiology
- Journal title:
- Journal of sports sciences
- Year:
- 2003
- Journal volume:
- 21
- Month:
- May
- Journal issue:
- 5
- Pages contribution:
- 383-9
- Fulltext / DOI:
- doi:10.1080/0264041031000071164
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/12800860
- Print-ISSN:
- 0264-0414 (Print) 0264-0414 (Linking)de
- TUM Institution:
- Department of Biomechanics in Sports
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