High accuracy of mesoscopic epi-fluorescence tomography for non-invasive quantitative volume determination of fluorescent protein-expressing tumours in mice.

Abou-Elkacem, Lotfi; Björn, Saskia; Doleschel, Dennis; Ntziachristos, Vasilis; Schulz, Ralf; Hoffman, Robert M; Kiessling, Fabian; Lederle, Wiltrud

doi:10.1007/s00330-012-2462-x

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Title:: High accuracy of mesoscopic epi-fluorescence tomography for non-invasive quantitative volume determination of fluorescent protein-expressing tumours in mice.
Document type:: Journal Article; Research Support, Non-U.S. Gov't; Article
Author(s):: Abou-Elkacem, Lotfi; Björn, Saskia; Doleschel, Dennis; Ntziachristos, Vasilis; Schulz, Ralf; Hoffman, Robert M; Kiessling, Fabian; Lederle, Wiltrud
Abstract:: To compare mesoscopic epi-fluorescence tomography (MEFT) and EPRI-illumination reflectance imaging (EPRI) for quantitative tumour size assessment in mice.Tumour xenografts of green/red fluorescent protein (GFP/RFP)-expressing colon cancer cells were measured using MEFT, EPRI, ultrasound (US) and micro computed tomography (?CT) at day 14 post-injection (n = 6). Results from MEFT and EPRI were correlated with each other and with US and ?CT (reference methods). Tumour volumes were measured ex vivo by GFP and RFP fluorescence imaging on cryoslices and compared with the in vivo measurements.High correlation and congruency were observed between MEFT, US and ?CT (MEFT/US: GFP: r (2) = 0.96; RFP: r (2) = 0.97, both P < 0.05; MEFT/?CT: GFP: r (2) = 0.93; RFP: r (2) = 0.90; both P < 0.05). Additionally, in vivo MEFT data were highly correlated and congruent with ex vivo cryoslice imaging results (GFP: r (2) = 0.96; RFP: r (2) = 0.99; both P < 0.05). In comparison, EPRI significantly overestimated tumour volumes (P < 0.05), although there was a significant correlation with US and ?CT (EPRI/US: GFP: r (2) = 0.95; RFP: r (2) = 0.94; both P < 0.05; EPRI/?CT GFP: r (2) = 0.86; RFP: r (2) = 0.86; both P < 0.05).Fluorescence distribution reconstruction using MEFT affords highly accurate three-dimensional (3D) tumour volume data showing superior accuracy compared to EPRI. Thus, MEFT is a very suitable technique for quantitatively assessing fluorescence distribution in superficial tumours at high spatial resolution.
Journal title abbreviation:: Eur Radiol
Year:: 2012
Journal volume:: 22
Journal issue:: 9
Pages contribution:: 1955-62
Language:: eng
Fulltext / DOI:: doi:10.1007/s00330-012-2462-x
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/22544295
Print-ISSN:: 0938-7994
TUM Institution:: Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)
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mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Preclinical Medicine Weitere Lehrstühle und Professuren Preclinical Medicine Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)2012