Dipeptidase 1 (DPEP1) is a marker for the transition from low-grade to high-grade intraepithelial neoplasia and an adverse prognostic factor in colorectal cancer.

Eisenach, PA; Soeth, E; Röder, C; Klöppel, G; Tepel, J; Kalthoff, H; Sipos, B

doi:10.1038/bjc.2013.363

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journal article

Title:: Dipeptidase 1 (DPEP1) is a marker for the transition from low-grade to high-grade intraepithelial neoplasia and an adverse prognostic factor in colorectal cancer.
Document type:: Journal Article; Research Support, Non-U.S. Gov't
Author(s):: Eisenach, PA; Soeth, E; Röder, C; Klöppel, G; Tepel, J; Kalthoff, H; Sipos, B
Abstract:: Colorectal cancer (CRC) is the second leading cause of cancer-related deaths worldwide. Improvements in the understanding of its molecular mechanism and the characterisation of CRC-specific biomarkers facilitating early detection are considered to increase overall survival.A meta-analysis of microarray and Serial Analysis of Gene Expression (SAGE) has been performed to identify differentially regulated genes in CRC. Dipeptidase 1 (DPEP1/MDP/RDP) and Syntenin-2 (SDCBP2/SITAC18) were found to be differentially expressed in tumour tissue compared with normal mucosa. Expression of DPEP1 was assessed in a validation set of 87 normal mucosa samples, 20 hyperplastic polyps, 46 CR adenomas with low- and high-grade intraepithelial neoplasia (IEN) and 217 well-documented CRCs by immunohistochemistry and partially by immunoblotting and real-time PCR.Expression of DPEP1 was specifically increased in human CRC tissue samples compared with normal mucosa (P<0.0001, Mann-Whitney U-test), showing a striking upregulation in high-grade compared with low-grade IEN. Furthermore, high DPEP1 expression was found to strongly correlate with histological stage (P<0.0001, chi-square test) as well as localisation (P<0.0001, chi-square test) and has been recognised as an independent adverse prognostic factor, showing significant prognostic values with an ROC (receiver operating characteristic)-AUC of 0.9230.Dipeptidase 1 has been identified as an excellent marker of high-grade IEN and CRC, and may thus be applied for screening of early neoplastic lesions and for prognostic stratification.
Journal title abbreviation:: Br J Cancer
Year:: 2013
Journal volume:: 109
Journal issue:: 3
Pages contribution:: 694-703
Language:: eng
Fulltext / DOI:: doi:10.1038/bjc.2013.363
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/23839495
Print-ISSN:: 0007-0920
TUM Institution:: Institut für Allgemeine Pathologie und Pathologische Anatomie
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mediaTUM Gesamtbestand Hochschulbibliographie 2013 Fakultäten Medizin Institut für Allgemeine Pathologie und Pathologische Anatomie

mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Preclinical Medicine Institut für Allgemeine Pathologie und Pathologische Anatomie (Dr. Mogler komm.)2013