Molecular imaging of early ?v?3 integrin expression predicts long-term left-ventricle remodeling after myocardial infarction in rats.

Sherif, HM; Saraste, A; Nekolla, SG; Weidl, E; Reder, S; Tapfer, A; Rudelius, M; Higuchi, T; Botnar, RM; Wester, HJ; Schwaiger, M

doi:10.2967/jnumed.111.091652

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Title:: Molecular imaging of early ?v?3 integrin expression predicts long-term left-ventricle remodeling after myocardial infarction in rats.
Document type:: Journal Article; Research Support, Non-U.S. Gov't
Author(s):: Sherif, HM; Saraste, A; Nekolla, SG; Weidl, E; Reder, S; Tapfer, A; Rudelius, M; Higuchi, T; Botnar, RM; Wester, HJ; Schwaiger, M
Abstract:: (18)F-galacto-RGD ((18)F-RGD) is a PET tracer binding to ?(v)?(3) integrin receptors that are upregulated after myocardial infarction (MI) as part of the healing process. We studied whether myocardial (18)F-RGD uptake early after MI is associated with long-term left-ventricle (LV) remodeling in a rat model.Wistar rats underwent sham operation (n = 9) or permanent coronary ligation (n = 25). One week after MI, rats were injected with (18)F-RGD to evaluate ?(v)?(3) integrin expression using a preclinical PET system. In the same rats, LV volumes and defect size were measured 1 and 12 wk after MI by (13)N-ammonia PET and MRI, respectively.One week after MI, (18)F-RGD uptake was increased in the defect area as compared with the remote myocardium of MI rats or sham-operated controls (percentage injected dose per cubic centimeter, 0.20 ± 0.05 vs. 0.06 ± 0.03 and 0.07 ± 0.04, P < 0.001). At this time, (18)F-RGD uptake was associated with capillary density in histologic sections. Average (18)F-RGD uptake in the defect area was lowest in the rats demonstrating greater than 20% relative increase in the LV end-diastolic volume from 1 to 12 wk (percentage injected dose per centimeter cubed, 0.15 ± 0.07 vs. 0.21 ± 0.05, P < 0.05). In a multivariable logistic regression analysis, low (18)F-RGD uptake was a significant predictor of increase in end-diastolic volume (r = 0.51, P < 0.05).High levels of (18)F-RGD uptake in the perfusion defect area early after MI were associated with the absence of significant LV remodeling after 12 wk of follow-up. These results suggest that ?(v)?(3) integrin expression is a potential biomarker of myocardial repair processes after MI and enables the monitoring of these processes by molecular imaging to derive possible prognostic information.
Journal title abbreviation:: J Nucl Med
Year:: 2012
Journal volume:: 53
Journal issue:: 2
Pages contribution:: 318-23
Language:: eng
Fulltext / DOI:: doi:10.2967/jnumed.111.091652
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/22302965
Print-ISSN:: 0161-5505
TUM Institution:: Institut für Allgemeine Pathologie und Pathologische Anatomie; Klinik und Poliklinik für Nuklearmedizin
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