Immunohistochemical analysis of osteoblasts in zygapophyseal joints of patients with ankylosing spondylitis reveal repair mechanisms similar to osteoarthritis.

Appel, H; Maier, R; Loddenkemper, C; Kayser, R; Meier, O; Hempfing, A; Sieper, J

doi:10.3899/jrheum.090986

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Title:: Immunohistochemical analysis of osteoblasts in zygapophyseal joints of patients with ankylosing spondylitis reveal repair mechanisms similar to osteoarthritis.
Document type:: Journal Article
Author(s):: Appel, H; Maier, R; Loddenkemper, C; Kayser, R; Meier, O; Hempfing, A; Sieper, J
Abstract:: OBJECTIVE: New bone formation of the spine is a typical feature of ankylosing spondylitis (AS). It is unknown whether new bone formation is part of a physiological repair process or a unique pathological entity of the disease. METHODS: We analyzed zygapophyseal joints from patients with AS and osteoarthritis (OA) undergoing spinal surgery for rigid hyperkyphosis (AS) or radiculopathy caused by severe OA. In 17 patients with AS, 11 with OA, and 5 controls we performed immunohistochemical analysis of osteoprotegerin (OPG), nuclear factor-kappaB ligand (RANKL), and osteocalcin (OC) expression in osteoblasts and determined the trabecular thickness in AS and OA patients and controls. Osteoclasts were detected by tartrate-resistant alkaline phosphatase (TRAP) staining. RESULTS: Trabecular thickness was significantly lower in patients with AS compared to OA (p = 0.01). The absolute number of CD56+ osteoblasts (p < 0.001) and OC+ (p = 0.002), OPG+ (p = 0.003), and RANKL+ osteoblasts (p = 0.03) in AS patients was also significantly lower than in OA patients. The percentages of OC+, OPG+, and RANKL+ osteoblasts did not differ between AS and OA (p > 0.05 in all cases). In controls, the percentages of OPG+ (p = 0.013) and OC+ (p = 0.034) but not RANKL+ (p > 0.05) osteoblasts were significantly lower compared to AS patients. The frequency of TRAP+ osteoclasts in AS patients was significantly lower compared to OA (p < 0.001), but higher compared to controls. CONCLUSION: Immunohistochemical analysis of zygapophyseal joints suggested that osteoblast activity is similar in AS and OA, indicating that new bone formation is possibly a physiological function of repair in both diseases.
Journal title abbreviation:: J Rheumatol
Year:: 2010
Journal volume:: 37
Journal issue:: 4
Pages contribution:: 823-8
Language:: eng
Fulltext / DOI:: doi:10.3899/jrheum.090986
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/20156950
Print-ISSN:: 0315-162X
TUM Institution:: Institut für Allgemeine Pathologie und Pathologische Anatomie
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mediaTUM Gesamtbestand Hochschulbibliographie 2010 Fakultäten Medizin Institut für Allgemeine Pathologie und Pathologische Anatomie

mediaTUM Gesamtbestand Einrichtungen Schools TUM School of Medicine and Health Departments Preclinical Medicine Institut für Allgemeine Pathologie und Pathologische Anatomie (Dr. Mogler komm.)2010