Ochratoxin A, one of the 5 most important mycotoxins in food safety, is produced by several Aspergillus and Penicillium strains growing on grain, grapes and coffee. OTA biosynthesis in Aspergillus spp. depends more on the complex interaction of diverse environmental conditions than on a single growth factor or even the genetic ability to produce OTA. No apparent connection between biomass and OTA production was observed. OTA production by Aspergillus ochraceus could be controlled by the pH of the culture medium, with accurately defined growth conditions applied. Analysis of cultures grown under OTA permissive conditions led to the identification of two differentially expressed cDNA fragments. Two primer pairs were derived from those fragments with different detection spectra for ochratoxigenic Aspergillus spp. Primer pair 3for / 3rev2 was suitable for specific detection of the species A. ochraceus. Primer pair 4for / 4rev2 was applicable for detection and discrimination of several ochratoxigenic species /strains within the A. ochraceus group and the A. carbonarius group.
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Ochratoxin A, one of the 5 most important mycotoxins in food safety, is produced by several Aspergillus and Penicillium strains growing on grain, grapes and coffee. OTA biosynthesis in Aspergillus spp. depends more on the complex interaction of diverse environmental conditions than on a single growth factor or even the genetic ability to produce OTA. No apparent connection between biomass and OTA production was observed. OTA production by Aspergillus ochraceus could be controlled by the pH of th...
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