Over a period of 25 months, lactating Fleckvieh cows were fed on either genetically modified maize (MON810, N=18) or the non-transgenic variety. Feces, blood and milk samples were taken monthwise, whereas urine sample were taken bimonthly and feed samples weekly. Aim of this study was to investigate the fate of the cry1Ab DNA and the novel protein, and analysis of the degradation pattern of the Cry1Ab protein in the gastrointestinal tract. Therefore, new ELISA, PCR and quantitative PCR assays were established and validated. The mean intake of cows in the group fed transgenic maize amounted to 6.0 mg Cry1Ab protein per cow daily. A transfer of transgenic components into blood, urine or milk was not detected. Only in feces of the transgenic fed group, the Cry1Ab protein was found. In comparison to other proteins in feed, the Cry1Ab protein underlies a faster degradation.
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Over a period of 25 months, lactating Fleckvieh cows were fed on either genetically modified maize (MON810, N=18) or the non-transgenic variety. Feces, blood and milk samples were taken monthwise, whereas urine sample were taken bimonthly and feed samples weekly. Aim of this study was to investigate the fate of the cry1Ab DNA and the novel protein, and analysis of the degradation pattern of the Cry1Ab protein in the gastrointestinal tract. Therefore, new ELISA, PCR and quantitative PCR assays we...
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