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Title:

Isolation of high quality protein samples from punches of formalin fixed and paraffin embedded tissue blocks.

Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article
Author(s):
Kroll, J; Becker, KF; Kuphal, S; Hein, R; Hofstädter, F; Bosserhoff, AK
Abstract:
In general, it is believed that the extraction of proteins from formalin-fixed paraffin embedded samples is not feasible. However, recently a new technique was developed, presenting the extraction of non-degraded, full length proteins from formalin fixed tissues, usable for western blotting and protein arrays. In the study presented here, we applied this technique to punch biopsies of formalin fixed tissues embedded in paraffin to reduce heterogeneity of the tissue represented in sections, and t...     »
Journal title abbreviation:
Histol Histopathol
Year:
2008
Journal volume:
23
Journal issue:
4
Pages contribution:
391-5
Language:
eng
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/18228195
Print-ISSN:
0213-3911
TUM Institution:
Institut für Allgemeine Pathologie und Pathologische Anatomie; Klinik und Poliklinik für Dermatologie und Allergologie
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