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Title:

Isolation of highly pure rat liver mitochondria with the aid of zone-electrophoresis in a free flow device (ZE-FFE).

Document type:
Journal Article; Review
Author(s):
Zischka, H; Lichtmannegger, J; Jägemann, N; Jennen, L; Hamöller, D; Huber, E; Walch, A; Summer, KH; Göttlicher, M
Abstract:
This protocol describes the purification of mitochondria from rat liver with the aid of zone electrophoresis in a free flow device (ZE-FFE). Starting from liver homogenate, cell debris and nuclei are removed by low speed centrifugation. A crude mitochondrial fraction is obtained by medium speed centrifugation and is further purified by washing followed by a Nycodenz gradient centrifugation. Lysosomes and microsomes are located at the upper parts of the gradient, whereas mitochondria are found in...     »
Journal title abbreviation:
Methods Mol Biol
Year:
2008
Journal volume:
424
Pages contribution:
333-48
Language:
eng
Fulltext / DOI:
doi:10.1007/978-1-60327-064-9_26
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/18369873
Print-ISSN:
1064-3745
TUM Institution:
Institut für Toxikologie und Umwelthygiene
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