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Title:

One-Vector System for Multiplexed CRISPR/Cas9 against Hepatitis B Virus cccDNA Utilizing High-Capacity Adenoviral Vectors.

Document type:
Journal Article
Author(s):
Schiwon, Maren; Ehrke-Schulz, Eric; Oswald, Andreas; Bergmann, Thorsten; Michler, Thomas; Protzer, Ulrike; Ehrhardt, Anja
Abstract:
High-capacity adenoviral vectors (HCAdVs) devoid of all coding genes are powerful tools to deliver large DNA cargos into cells. Here HCAdVs were designed to deliver a multiplexed complete CRISPR/Cas9 nuclease system or a complete pair of transcription activator-like effector nucleases (TALENs) directed against the hepatitis B virus (HBV) genome. HBV, which remains a serious global health burden, forms covalently closed circular DNA (cccDNA) as a persistent DNA species in infected cells. This ccc...     »
Journal title abbreviation:
Mol Ther Nucleic Acids
Year:
2018
Journal volume:
12
Pages contribution:
242-253
Language:
eng
Fulltext / DOI:
doi:10.1016/j.omtn.2018.05.006
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/30195763
TUM Institution:
Institut für Virologie
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