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Title:

Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection.

Document type:
Journal Article
Author(s):
Schneider, Annika; Kurz, Sandra; Manske, Katrin; Janas, Marianne; Heikenwälder, Mathias; Misgeld, Thomas; Aichler, Michaela; Weissmann, Sebastian Felix; Zischka, Hans; Knolle, Percy; Wohlleber, Dirk
Abstract:
Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during vir...     »
Journal title abbreviation:
Sci Rep
Year:
2019
Journal volume:
9
Journal issue:
1
Fulltext / DOI:
doi:10.1038/s41598-019-44922-9
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/31186476
Print-ISSN:
2045-2322
TUM Institution:
Institut für Toxikologie und Umwelthygiene; Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld); Roman Herzog Comprehensive Cancer Center
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