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Title:

Isolation and Electron Microscopic Analysis of Liver Cancer Cell Mitochondria.

Document type:
Journal Article
Author(s):
Kabiri, Yaschar; Eberhagen, Carola; Schmitt, Sabine; Knolle, Percy A; Zischka, Hans
Abstract:
Isolation of mitochondria is a crucial method for examining molecular details of this organelle's manifold functions. Historically, mitochondrial isolations required large amounts of sample material which impeded their isolation from cultured cells. We have therefore developed a method allowing for controlled and reproducible isolation of intact and functional mitochondria from diverse cell types in culture. Here we provide a methodological update of this approach together with a protocol for th...     »
Journal title abbreviation:
Methods Mol Biol
Year:
2021
Journal volume:
2277
Pages contribution:
277-287
Fulltext / DOI:
doi:10.1007/978-1-0716-1270-5_17
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/34080157
Print-ISSN:
1064-3745
TUM Institution:
Institut für Toxikologie und Umwelthygiene; Roman Herzog Comprehensive Cancer Center
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