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Title:

Genome-wide off-target analyses of CRISPR/Cas9-mediated T-cell receptor engineering in primary human T cells.

Document type:
Article; Journal Article
Author(s):
Kaeuferle, Theresa; Stief, Tanja A; Canzar, Stefan; Kutlu, Nayad N; Willier, Semjon; Stenger, Dana; Ferrada-Ernst, Paulina; Habjan, Nicola; Peters, Annika E; Busch, Dirk H; Feuchtinger, Tobias
Abstract:
OBJECTIVES: Exploiting the forces of human T cells for treatment has led to the current paradigm of emerging immunotherapy strategies. Genetic engineering of the T-cell receptor (TCR) redirects specificity, ablates alloreactivity and brings significant progress and off-the-shelf options to emerging adoptive T-cell transfer (ACT) approaches. Targeted CRISPR/Cas9-mediated double-strand breaks in the DNA enable knockout or knock-in engineering. METHODS: Here, we perform CRISPR/Cas9-mediated TCR kno...     »
Journal title abbreviation:
Clin Transl Immunology
Year:
2022
Journal volume:
11
Journal issue:
1
Fulltext / DOI:
doi:10.1002/cti2.1372
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/35106156
TUM Institution:
611; Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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