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Title:

SARS-CoV-2 CD8+ T cell killing assays using replicating viruses and transgenic antigens.

Document type:
Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Mateyka, Laura M; Grass, Vincent; Pichlmair, Andreas; Busch, Dirk H; D'Ippolito, Elvira
Abstract:
The quality of an antigen-specific CD8+ T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8+ T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For complete details on the u...     »
Journal title abbreviation:
STAR Protoc
Year:
2022
Journal volume:
3
Journal issue:
4
Fulltext / DOI:
doi:10.1016/j.xpro.2022.101699
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/36152303
TUM Institution:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene (Prof. Busch); Institut für Virologie (Prof. Protzer)
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