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Titel:

An Evaluation of T-Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation.

Dokumenttyp:
Journal Article
Autor(en):
Andrä, Immanuel; Ulrich, Hanna; Dürr, Susi; Soll, Dominik; Henkel, Lynette; Angerpointner, Corinne; Ritter, Julia; Przibilla, Sabine; Stadler, Herbert; Effenberger, Manuel; Busch, Dirk H; Schiemann, Matthias
Abstract:
Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functio...     »
Zeitschriftentitel:
Cytometry A
Jahr:
2020
Band / Volume:
97
Heft / Issue:
2
Seitenangaben Beitrag:
171-183
Volltext / DOI:
doi:10.1002/cyto.a.23964
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/31944553
Print-ISSN:
1552-4922
TUM Einrichtung:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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