One therapeutic approach is based on the vaccination of cancer patients with inactivated autologous tumour cells. Aim of this work was to test the treatment of tumourcells with high hydrostatic pressure (HHP) > 100 MPa as an alternative technique to inactivate cell based vaccines. We analyzed the matter of cell death, the efficacy of the inactivation, and the immunogenicity of cells treated applying HHP. At 200 MPa, cells died by apoptosis. Furthermore cells pressurized above 300 MPa necrotized during treatment. Most importantly, both pressurized apoptotic and necrotic cells retained their immunogenicity in vivo.     «

One therapeutic approach is based on the vaccination of cancer patients with inactivated autologous tumour cells. Aim of this work was to test the treatment of tumourcells with high hydrostatic pressure (HHP) > 100 MPa as an alternative technique to inactivate cell based vaccines. We analyzed the matter of cell death, the efficacy of the inactivation, and the immunogenicity of cells treated applying HHP. At 200 MPa, cells died by apoptosis. Furthermore cells pressurized above 300 MPa necrotized...     »