The use of livestock animals to model human cancers depends on the ability to control expression of mutated oncogenes or tumour suppressor genes in a spatial- and temporal-specific manner. In this work, different approaches to create pancreas-,lung- and gut-specific Cre-driver pig lines for cancer research were investigated, using PDX-1, SFTP-C and villin-1 promoters, respectively. Also, aiming at controlling the Cre activity in a temporal manner, 2 tamoxifen-inducible forms of Cre were compared in vitro.      «

The use of livestock animals to model human cancers depends on the ability to control expression of mutated oncogenes or tumour suppressor genes in a spatial- and temporal-specific manner. In this work, different approaches to create pancreas-,lung- and gut-specific Cre-driver pig lines for cancer research were investigated, using PDX-1, SFTP-C and villin-1 promoters, respectively. Also, aiming at controlling the Cre activity in a temporal manner, 2 tamoxifen-inducible forms of Cre were compared...     »