Pigs carrying an APC mutation orthologous to the human APC¹³⁰⁹ mutation frequently diagnosed in human colorectal cancer were characterised by mRNA and miRNA sequencing to identify modifier genes of severe polyposis and genes driving tumour progression. The gene CYP7A1, miRNAs miR-215 and 194b-5p were associated with severe polyposis and the genes PLXND1 and GBP6 and miRNAs let-7e, miR-146a-5p, 146b, 183, 196a with tumour progression. The CAS9 gene was placed at the ROSA26 locus of APC¹³¹¹ cells to generate cloned piglets, in which gene editing can then be carried out directly in the polyps to accelerate tumour progression.     «

Pigs carrying an APC mutation orthologous to the human APC¹³⁰⁹ mutation frequently diagnosed in human colorectal cancer were characterised by mRNA and miRNA sequencing to identify modifier genes of severe polyposis and genes driving tumour progression. The gene CYP7A1, miRNAs miR-215 and 194b-5p were associated with severe polyposis and the genes PLXND1 and GBP6 and miRNAs let-7e, miR-146a-5p, 146b, 183, 196a with tumour progression. The CAS9 gene was placed at the ROSA26 locus of APC¹³¹¹ cells...     »