This works main goal was to study strategies for the molecular and enzymatic characterisation of new solvent-producing mesophylic Clostridium isolates from Colombia and ascertain their solvent producing biotechnological potential by using a cheap agro-industrial waste as carbon source. Molecular characterisation of the native strains using 16S rRNA, PFGE and DNA- DNA hybridisation shown that the native strains are closely related to each other and not belong to Clostridium butyricum and suggested that the native strains could be new specie. The IBUN 22A strain presented greater polysaccharide hydrolysing or degrading capacity within those native strains evaluated in POME Palm Oil mill efluent Medium, according to the quantity of acid produced. The IBUN IV and IBUN 22A strains showed acidogenic fermentation characteristics in POME Medium during the kinetic of growth. Selected strains will be used for biomass conversion to solvents, the fermentation process will be optimised and to contribute with the environmental protection.
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This works main goal was to study strategies for the molecular and enzymatic characterisation of new solvent-producing mesophylic Clostridium isolates from Colombia and ascertain their solvent producing biotechnological potential by using a cheap agro-industrial waste as carbon source. Molecular characterisation of the native strains using 16S rRNA, PFGE and DNA- DNA hybridisation shown that the native strains are closely related to each other and not belong to Clostridium butyricum and suggest...
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