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Title:

Using reflectometry to minimize the dependence of fluorescence intensity on optical absorption and scattering.

Document type:
Zeitschriftenaufsatz
Author(s):
Arias, Augusto; Anastasopoulou, Maria; Gorpas, Dimitris; Ntziachristos, Vasilis
Abstract:
The total diffuse reflectance RT and the effective attenuation coefficient µeff of an optically diffuse medium map uniquely onto its absorption and reduced scattering coefficients. Using this premise, we developed a methodology where RT and the slope of the logarithmic spatially resolved reflectance, a quantity related to µeff, are the inputs of a look-up table to correct the dependence of fluorescent signals on the media's optical properties. This methodology does not require an estimation of t...     »
Journal title:
Biomedical Optics Express
Journal title abbreviation:
Biomed Opt Express
Year:
2023
Journal volume:
14
Journal issue:
10
Pages contribution:
5499-5511
Fulltext / DOI:
doi:10.1364/BOE.496599
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/37854563
WWW:
https://opg.optica.org/boe/fulltext.cfm?uri=boe-14-10-5499&id=540350
Print-ISSN:
2156-7085
Copyright statement:
© 2023 Optica Publishing Group. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
TUM Institution:
Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)
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