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Title:

Development of a Global Metabo-Lipid-Prote-omics Workflow to Compare Healthy Proximal and Distal Colonic Epithelium in Mice

Document type:
Zeitschriftenaufsatz
Author(s):
Hemmati, Maryam; Wudy, Susanne I.; Hackbarth, Franziska; Mittermeier-Kleßinger, Verena K.; Coleman, Olivia I.; Haller, Dirk; Ludwig, Christina; Dawid, Corinna; Kleigrewe, Karin
Abstract:
A multimetabo-lipid-prote-omics workflow was developed to characterize the molecular interplay within proximal (PC) and distal (DC) colonic epithelium of healthy mice. This multiomics data set lays the foundation to better understand the two tissue types and can be used to study, for example, colon-related diseases like colorectal cancer or inflammatory bowel disease. First, the methyl tert-butyl ether extraction method was optimized, so that from a single tissue biopsy >350 reference-matched me...     »
Keywords:
BayBioMS; metabo-lipid-proteomics; methyl tert-butyl ether; mouse proximal and distal colons; multiomics.
Journal title:
Journal of Proteome Research
Year:
2024
Fulltext / DOI:
doi:10.1021/acs.jproteome.3c00771
Publisher:
American Chemical Society (ACS)
E-ISSN:
1535-38931535-3907
Date of publication:
25.07.2024
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