Oleaginous yeasts are reported as promising renewable feedstocks to replace plant sources and mineral oils in many applications by converting biomass to value-added material. Therefore, five prominent oleaginous yeast strains were analysed in complex hydrolysates of marine and terrestrial origins. Moreover, genetic tractability allows for flexible strain optimization via metabolic engineering. Hence, an efficient, flexible CRIPSR/Cas mediated genetic engineering technique featuring a plasmid-free transient Cas9 delivery strategy was developed for C. oleaginosus.
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Oleaginous yeasts are reported as promising renewable feedstocks to replace plant sources and mineral oils in many applications by converting biomass to value-added material. Therefore, five prominent oleaginous yeast strains were analysed in complex hydrolysates of marine and terrestrial origins. Moreover, genetic tractability allows for flexible strain optimization via metabolic engineering. Hence, an efficient, flexible CRIPSR/Cas mediated genetic engineering technique featuring a plasmid-fre...
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