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Titel:

Tutorial: design and execution of CRISPR in vivo screens.

Dokumenttyp:
Journal Article; Review; Research Support, Non-U.S. Gov't
Autor(en):
Braun, Christian J; Adames, Andrés Carbonell; Saur, Dieter; Rad, Roland
Abstract:
Here we provide a detailed tutorial on CRISPR in vivo screening. Using the mouse as the model organism, we introduce a range of CRISPR tools and applications, delineate general considerations for 'transplantation-based' or 'direct in vivo' screening design, and provide details on technical execution, sequencing readouts, computational analyses and data interpretation. In vivo screens face unique pitfalls and limitations, such as delivery issues or library bottlenecking, which must be counteracte...     »
Zeitschriftentitel:
Nat Protoc
Jahr:
2022
Band / Volume:
17
Heft / Issue:
9
Seitenangaben Beitrag:
1903-1925
Volltext / DOI:
doi:10.1038/s41596-022-00700-y
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/35840661
Print-ISSN:
1754-2189
TUM Einrichtung:
611; Lehrstuhl für Translationale Tumorforschung (DKTK) (Prof. Saur); Professur für Molekulare Onkologie und Funktionelle Genomik (Prof. Rad)
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