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Titel:

SARS-CoV-2 CD8+ T cell killing assays using replicating viruses and transgenic antigens.

Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't
Autor(en):
Mateyka, Laura M; Grass, Vincent; Pichlmair, Andreas; Busch, Dirk H; D'Ippolito, Elvira
Abstract:
The quality of an antigen-specific CD8+ T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8+ T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For complete details on the u...     »
Zeitschriftentitel:
STAR Protoc
Jahr:
2022
Band / Volume:
3
Heft / Issue:
4
Volltext / DOI:
doi:10.1016/j.xpro.2022.101699
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/36152303
TUM Einrichtung:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene (Prof. Busch); Institut für Virologie (Prof. Protzer)
 BibTeX