Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons.

Title:: Live-Cell Imaging of RNA Transport in Axons of Cultured Primary Neurons.
Document type:: Journal Article
Author(s):: Hees, J Tabitha; Harbauer, Angelika B
Abstract:: The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging.
Journal title abbreviation:: Methods Mol Biol
Year:: 2022
Journal volume:: 2431
Pages contribution:: 225-237
Fulltext / DOI:: doi:10.1007/978-1-0716-1990-2_11
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/35412279
Print-ISSN:: 1064-3745
TUM Institution:: Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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