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Title:

Identification of 7 000–9 000 Proteins from Cell Lines and Tissues by Single-Shot Microflow LC–MS/MS

Document type:
Zeitschriftenaufsatz
Author(s):
Bian, Yangyang; The, Matthew; Giansanti, Piero; Mergner, Julia; Zheng, Runsheng; Wilhelm, Mathias; Boychenko, Alexander; Kuster, Bernhard
Abstract:
A current trend in proteomics is to acquire data in a "single-shot" by LC-MS/MS because it simplifies workflows and promises better throughput and quantitative accuracy than schemes that involve extensive sample fractionation. However, single-shot approaches can suffer from limited proteome coverage when performed by data dependent acquisition (ssDDA) on nanoflow LC systems. For applications where sample quantities are not scarce, this study shows that high proteome coverage can be obtained usin...     »
Journal title:
Analytical Chemistry
Year:
2021
Journal volume:
93
Journal issue:
25
Pages contribution:
8687-8692
Fulltext / DOI:
doi:10.1021/acs.analchem.1c00738
Publisher:
American Chemical Society (ACS)
E-ISSN:
0003-27001520-6882
Date of publication:
14.06.2021
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