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Title:

Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells.

Document type:
Zeitschriftenaufsatz
Author(s):
Verbelen, Benno; Girardi, Tiziana; Sulima, Sergey O; Vereecke, Stijn; Verstraete, Paulien; Verbeeck, Jelle; Royaert, Jonathan; Cinque, Sonia; Montanaro, Lorenzo; Penzo, Marianna; Imbrechts, Maya; Geukens, Nick; Geuens, Thomas; Dierckx, Koen; Pepe, Daniele; Kampen, Kim; De Keersmaecker, Kim
Abstract:
Mammalian cells are commonly used to produce recombinant protein therapeutics, but suffer from a high cost per mg of protein produced. There is therefore great interest in improving protein yields to reduce production cost. We present an entirely novel approach to reach this goal through direct engineering of the cellular translation machinery by introducing the R98S point mutation in the catalytically essential ribosomal protein L10 (RPL10-R98S). Our data support that RPL10-R98S enhances transl...     »
Journal title:
Engineering in Life Sciences
Journal title abbreviation:
ENGINEERING IN LIFE SCIENCES
Year:
2022
Journal volume:
22
Journal issue:
2
Pages contribution:
100-114
Fulltext / DOI:
doi:10.1002/elsc.202100124
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/35140557
WWW:
https://onlinelibrary.wiley.com/doi/10.1002/elsc.202100124
Print-ISSN:
1618-0240
TUM Institution:
Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)
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