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Title:

Gene Editing in Primary Cells of Cattle and Pig.

Document type:
Journal Article
Author(s):
Vochozkova, Petra; Simmet, Kilian; Jemiller, Eva-Maria; Wünsch, Annegret; Klymiuk, Nikolai
Abstract:
Gene Editing by CRISPR/Cas has revolutionized many aspects of biotechnology within a short period of time. This is also true for the genetic manipulation of livestock species, but their specific challenges such as the lack of stem cells, the limited proliferative capacity of primary cells, and the genetic diversity of the pig and cattle populations need consideration when CRISPR/Cas is applied. Here we present guidelines for CRISPRing primary cells in pig and cattle, with a specific focus on tes...     »
Journal title abbreviation:
Methods Mol Biol
Year:
2019
Journal volume:
1961
Pages contribution:
271-289
Fulltext / DOI:
doi:10.1007/978-1-4939-9170-9_17
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/30912052
Print-ISSN:
1064-3745
TUM Institution:
1693
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