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Title:

Dissociation of microdissected mouse brain tissue for artifact free single-cell RNA sequencing.

Document type:
Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Liu, Lu; Besson-Girard, Simon; Ji, Hao; Gehring, Katrin; Bulut, Buket; Kaya, Tuğberk; Usifo, Fumere; Simons, Mikael; Gokce, Ozgun
Abstract:
Single-cell RNA sequencing (scRNA-seq) provides the transcriptome of individual cells and addresses previously intractable problems including the central nervous system's transcriptional responses during health and disease. However, dissociating brain cells is challenging and induces artificial transcriptional responses. Here, we describe an enzymatic dissociation method for mouse brain that prevents dissociation artifacts and lowers technical variations with standardized steps. We tested this p...     »
Journal title abbreviation:
STAR Protoc
Year:
2021
Journal volume:
2
Journal issue:
2
Fulltext / DOI:
doi:10.1016/j.xpro.2021.100590
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/34159323
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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