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Title:

Generation and detection of S-nitrosothiols.

Document type:
Zeitschriftenaufsatz
Author(s):
Lindermayr, Christian; Sell, Simone; Durner, Jorg
Abstract:
Nitric oxide (NO) plays a pivotal role in cellular signaling in many different organisms as the result of the modification of protein activities/functions by protein S-nitrosylation. This NO-dependent posttranslational modification is based on the attachment of NO to the sulfur moiety of cysteine residues. However, the instability of S-nitrosothiols makes it difficult to analyze this type of protein modification in vitro as well as in vivo. Jeffrey and colleagues developed a method--named the bi...     »
Keywords:
Arabidopsis/cytology/metabolism, Arabidopsis Proteins/analysis/chemistry, Avidin/metabolism, Biotinylation, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Molecular Biology/*methods, Nitrosation, Protein Structure, Secondary, S-Nitrosothiols/*analysis
Journal title:
Methods in molecular biology (Clifton, N.J.)
Year:
2008
Journal volume:
476
Pages contribution:
217-29
Language:
eng
Status:
MEDLINE
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