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Title:

cDNA-directed expression of human cytochrome P450 CYP3A4 using baculovirus.

Document type:
Journal Article
Author(s):
Buters, J T; Korzekwa, K R; Kunze, K L; Omata, Y; Hardwick, J P; Gonzalez, F J
Abstract:
A recombinant baculovirus containing the human CYP3A4 cDNA was constructed and used to express CYP3A4 in SF9 insect cells (0.46 +/- 0.13 nmol/mg protein, 103 +/- 29 nmol/liter, N = 15). The enzyme represented approximately 2-3% of total cellular protein and could be purified by a two-column procedure to a specific content of 12.7 nmol/mg protein. Catalytic activity of the purified enzyme after reconstitution was optimum using molar ratios of CYP3A4 to cytochrome b5 to NADPH-P450 oxidoreductase o...     »
Journal title abbreviation:
Drug Metab Dispos
Year:
1994
Journal volume:
22
Journal issue:
5
Pages contribution:
688-92
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/7835218
Print-ISSN:
0090-9556
TUM Institution:
Molekulare Allergologie (Prof. Schmidt-Weber)
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