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Title:

CRISPR-Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV-host factor interactions.

Document type:
Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Hultquist, Judd F; Hiatt, Joseph; Schumann, Kathrin; McGregor, Michael J; Roth, Theodore L; Haas, Paige; Doudna, Jennifer A; Marson, Alexander; Krogan, Nevan J
Abstract:
CRISPR-Cas9 gene-editing strategies have revolutionized our ability to engineer the human genome for robust functional interrogation of complex biological processes. We have recently adapted this technology for use in primary human CD4+ T cells to create a high-throughput platform for analyzing the role of host factors in HIV infection and pathogenesis. Briefly, CRISPR-Cas9 ribonucleoproteins (crRNPs) are synthesized in vitro and delivered to activated CD4+ T cells by nucleofection. These cells...     »
Journal title abbreviation:
Nat Protoc
Year:
2019
Journal volume:
14
Journal issue:
1
Pages contribution:
1-27
Fulltext / DOI:
doi:10.1038/s41596-018-0069-7
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/30559373
Print-ISSN:
1754-2189
TUM Institution:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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