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Title:

Isolation and Purification of Mitochondria from Cell Culture for Proteomic Analyses.

Document type:
Journal Article
Author(s):
Kabiri, Yaschar; von Toerne, Christine; Fontes, Adriana; Knolle, Percy A; Zischka, Hans
Abstract:
In-depth analysis of the mitochondrial proteome can be greatly improved by analyzing isolated mitochondria instead of whole cells. However, isolation of sufficient amounts of mitochondria from cell culture has proven to be notoriously difficult due to small sample size. Thus, we have developed a reproducible, controllable, and highly customizable method to isolate high microgram to low milligram amounts of intact mitochondria from cell culture samples along with an optional density gradient puri...     »
Journal title abbreviation:
Methods Mol Biol
Year:
2021
Journal volume:
2261
Pages contribution:
411-419
Fulltext / DOI:
doi:10.1007/978-1-0716-1186-9_25
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/33421004
Print-ISSN:
1064-3745
TUM Institution:
Institut für Toxikologie und Umwelthygiene; Roman Herzog Comprehensive Cancer Center
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