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Titel:

Isolation and Purification of Mitochondria from Cell Culture for Proteomic Analyses.

Dokumenttyp:
Journal Article
Autor(en):
Kabiri, Yaschar; von Toerne, Christine; Fontes, Adriana; Knolle, Percy A; Zischka, Hans
Abstract:
In-depth analysis of the mitochondrial proteome can be greatly improved by analyzing isolated mitochondria instead of whole cells. However, isolation of sufficient amounts of mitochondria from cell culture has proven to be notoriously difficult due to small sample size. Thus, we have developed a reproducible, controllable, and highly customizable method to isolate high microgram to low milligram amounts of intact mitochondria from cell culture samples along with an optional density gradient puri...     »
Zeitschriftentitel:
Methods Mol Biol
Jahr:
2021
Band / Volume:
2261
Seitenangaben Beitrag:
411-419
Volltext / DOI:
doi:10.1007/978-1-0716-1186-9_25
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/33421004
Print-ISSN:
1064-3745
TUM Einrichtung:
Institut für Toxikologie und Umwelthygiene; Roman Herzog Comprehensive Cancer Center
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